Coding

Part:BBa_I756000:Design

Designed by: Andy Mendelsohn   Group: iGEM07_Bay_Area_RSI   (2007-05-26)


human DOCK11


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2068
    Illegal PstI site found at 1798
    Illegal PstI site found at 2126
    Illegal PstI site found at 2906
    Illegal PstI site found at 3340
    Illegal PstI site found at 5840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2068
    Illegal NheI site found at 3145
    Illegal NheI site found at 5977
    Illegal PstI site found at 1798
    Illegal PstI site found at 2126
    Illegal PstI site found at 2906
    Illegal PstI site found at 3340
    Illegal PstI site found at 5840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2068
    Illegal BglII site found at 249
    Illegal BglII site found at 2535
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2068
    Illegal PstI site found at 1798
    Illegal PstI site found at 2126
    Illegal PstI site found at 2906
    Illegal PstI site found at 3340
    Illegal PstI site found at 5840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2068
    Illegal PstI site found at 1798
    Illegal PstI site found at 2126
    Illegal PstI site found at 2906
    Illegal PstI site found at 3340
    Illegal PstI site found at 5840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 41
    Illegal BsaI.rc site found at 465
    Illegal SapI site found at 1352
    Illegal SapI site found at 1391
    Illegal SapI site found at 3439
    Illegal SapI site found at 4126


Design Notes

Could be used for polarity


Source

cDNA sequence http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=145699122&from=64&to=6285&view=gbwithparts

References